Amorphization-Modulated Metal Sulfides with Boosted Active Sites and Kinetics for Efficient Enzymatic Colorimetric Biodetection

Colorimetric biosensing has become a popular sensing method for the portable detection of a variety of biomarkers. Artificial biocatalysts can replace traditional natural enzymes in the fields of enzymatic colorimetric biodetection; however, the exploration of new biocatalysts with efficient, stable, and specific biosensing reactions has remained challenging so far. Here, to enhance the active sites and overcome the sluggish kinetics of metal sulfides, the creation of an amorphous RuS₂ (a-RuS₂ ) biocatalytic system is reported, which can dramatically boost the peroxidase-mimetic activity of RuS₂ for the enzymatic detection of diverse biomolecules. Due to the existence of abundant accessible active sites and mildly surface oxidation, the a-RuS₂ biocatalyst displays a twofold V_max value and much higher reaction kinetics/turnover number (1.63 × 10^-2 s^-1 ) compared to that of the crystallized RuS₂ . Noticeably, the a-RuS₂ -based biosensor shows an extremely low detection limit of H₂ O₂ (3.25 × 10^-6 m), l-cysteine (3.39 × 10^-6 m), and glucose (9.84 × 10^-6 m), respectively, thus showing superior detection sensitivity to many currently reported peroxidase-mimetic nanomaterials. This work offers a new path to create highly sensitive and specific colorimetric biosensors in detecting biomolecules and also provides valuable insights for engineering robust enzyme-like biocatalysts via amorphization-modulated design.