Ramlibacter paludis sp. nov., isolated from wetland

A Gram-stain-negative, non-motile, rod-shaped, aerobic and white-coloured bacterium (designated XY19^T) was isolated from a soil sample of wetland from Godeok Ecological Park, Gangdong-gu, Seoul, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain XY19^T clustered with species of the genus Ramlibacter and appeared closely related to R. ginsenosidimutans DSM 23480^T (98.42 %), R. alkalitolerans JCM 32081^T (97.68 %) and R. monticola JCM 31918^T (97.66 %). The average nucleotide identity between strain XY19^T and three strains (R. ginsenosidimutans DSM 23480^T, R. alkalitolerans JCM 32081^T and R. monticola JCM 31918^T) were 80.7, 81.1 and 81.4 %. And the digital DNA-DNA hybridization (dDDH) calculated between strain XY19^T and each of the three strains (R. ginsenosidimutans DSM 23480^T, R. alkalitolerans JCM 32081^T and R. monticola JCM 31918^T) were 24.1, 24.4 and 24.5 %. ANI value and dDDH results were a novel species of the genus Ramlibacter. Growth occurs at 10-37 °C on R2A medium in the pressence of 0-1 % NaCl (w/v) and at pH 6.0-8.5. The DNA G+C content of the genomic DNA was 68.7 mol%, and ubiquinone-8 (Q-8) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C_16:1 ω7c and/or C_16:1 ω6c (summed feature 3), C_{16 : 0}, C_{17 : 0} cyclo and C_18:1 ω7c and/or C_18:1 ω6c (summed feature 8). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified lipids and unidentified aminophospholipid. Physiological and biochemical characteristics indicated that strain XY19^T represents a novel species of the genus Ramlibacter, for which the name Ramlibacter paludis sp. nov. is proposed. The type strain is XY19^T (= KACC 22220^T = LMG 32190^T).