Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
J Zhejiang Univ Sci B. 2023 May 15;24(5):418-429.
doi: 10.1631/jzus.B2200440.
[Article in
English,
Chinese]
Affiliations
- 1 State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China.
- 2 Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China.
- 3 State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China. [email protected].
- 4 Bioland Laboratory, Guangzhou 510005, China. [email protected].
- 5 Guangzhou Laboratory, Guangzhou 510005, China. [email protected].
- 6 State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China. [email protected].
- 7 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. [email protected].
- 8 Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China. [email protected].
Efforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human primate cells. Here, we introduce a multiplexed single-cell sequencing technique to profile the molecular features of monkey pluripotent stem cells in published culture conditions. The results demonstrate suboptimized maintenance of pluripotency and show that the selected signaling pathways for resetting human stem cells can also be interpreted for establishing monkey cell lines. Overall, this work legitimates the translation of novel human cell line culture conditions to monkey cells and provides guidance for exploring chemical cocktails for monkey stem cell line derivation.
尽管人们已经开发了多种构建人类多能干细胞的方法，但这些方法仍未应用在非人灵长类细胞中。在本文中，我们利用多样品标记单细胞测序技术对不同培养条件下的猴多能干细胞进行了分子特征的表征。结果表明，虽然用于重置人类干细胞的信号通路可以用来构建猴干细胞系，但其配方有待优化才能更好地维持猴干细胞多能性。总而言之，本研究证实了新型的人类干细胞培养体系可以转化到猴干细胞体系中，这对于未来猴干细胞系培养配方的探索具有指导意义。.
尽管人们已经开发了多种构建人类多能干细胞的方法，但这些方法仍未应用在非人灵长类细胞中。在本文中，我们利用多样品标记单细胞测序技术对不同培养条件下的猴多能干细胞进行了分子特征的表征。结果表明，虽然用于重置人类干细胞的信号通路可以用来构建猴干细胞系，但其配方有待优化才能更好地维持猴干细胞多能性。总而言之，本研究证实了新型的人类干细胞培养体系可以转化到猴干细胞体系中，这对于未来猴干细胞系培养配方的探索具有指导意义。
Keywords:
Extended pluripotent stem cells; Monkey pluripotent stem cell; Multiplexed single-cell sequencing; Naive pluripotency.
MeSH terms
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Animals
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Cell Differentiation
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Cell Line
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Haplorhini
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Pluripotent Stem Cells* / metabolism
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Signal Transduction
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Single-Cell Gene Expression Analysis*
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Transcriptome