A tetranuclear polypyridylruthenium(II) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium

Kartika Wardhani; Aviva Levina; Biyun Sun; Georges E R Grau; F Richard Keene; J Grant Collins; Peter A Lay

doi:10.1039/d2cc05827h

A tetranuclear polypyridylruthenium(II) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium

Chem Commun (Camb). 2023 Jun 1;59(45):6877-6880. doi: 10.1039/d2cc05827h.

Authors

Kartika Wardhani¹, Aviva Levina¹, Biyun Sun², Georges E R Grau^{3

4

5

6}, F Richard Keene^{7

8}, J Grant Collins⁹, Peter A Lay^{1

3

4

5

10}

Affiliations

¹ School of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia. [email protected].
² Dementia Research Centre, Department of Biomedical Science, Macquarie University, Sydney, NSW, 2109, Australia.
³ Sydney Nano, The University of Sydney, Sydney, NSW, 2006, Australia.
⁴ Sydney Cancer Network, The University of Sydney, Sydney, NSW, 2006, Australia.
⁵ Marie Bashir Institute, The University of Sydney, Sydney, NSW, 2006, Australia.
⁶ Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, 2006, Australia.
⁷ Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide, SA, 5005, Australia.
⁸ Australian Institute of Tropical Health and Medicine/Centre for Molecular Therapeutics, James Cook University, Townsville, QLD, 4811, Australia.
⁹ School of Science, The University of New South Wales, Australian Defence Force Academy, Canberra, ACT, 2612, Australia.
¹⁰ Sydney Analytical, The University of Sydney, Sydney, NSW, 2006, Australia.

PMID: 37195631
DOI: 10.1039/d2cc05827h

Abstract

A new photoluminescent polypyridylruthenium(II) stain for extracellular vesicles (EVs) released from lipopolysaccharide-stimulated THP-1 monocytes enabled important new insights into how the bacteria-induced immune system affects the blood-brain barrier (BBB). These included previously unknown aspects of EV interactions with BBB microvascular endothelial cells and the extracellular matrix relevant to human brain diseases.

MeSH terms

Blood-Brain Barrier
Brain
Endothelial Cells*
Endothelium
Extracellular Vesicles*
Humans