Application of Matrigel in the 3-dimension culture of female germline stem cells

Female germline stem cells (FGSCs) are a group of rare undifferentiated cells found in ovarian cortex, which have the unique ability to self-renew and differentiate. Stable maintenance and proliferation of FGSCs in culture are pivotal for clinic research. However, conventional 2-D (dimension) culture systems are limited in their ability to mimic the ovarian microenvironment during in vitro studies. To establish a suitable in vitro microenvironment for FGSCs, we conducted experiments using a Matrigel-based 3-D culture system. This involves testing different dilution ratios, medium compositions, and co-culture cells to find the optimal conditions for FGSCs maintenance and proliferation. Our results demonstrated the feasibility of using Matrigel as a FGSCs 3-D culture media. Moreover, co-culturing FGSCs with some types of cells in the Matrigel-based 3-D culture system had the potential to form ovarian organoids. Meanwhile, the safety of Matrigel was confirmed in vivo through transplantation experiment, which suggests the potential for clinic research.