1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

Srdan Matosin; Patrick D Fischer; Maxim A Droemer; Eric Baggs; Abu Sayeed Chowdhury; Isidoro Tavares; Scott B Ficarro; Lisa Rose Warner; Haribabu Arthanari; Rajesh Nagarajan

doi:10.1007/s12104-023-10136-4

¹H, ¹³C and ¹⁵N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

Biomol NMR Assign. 2023 Dec;17(2):167-171. doi: 10.1007/s12104-023-10136-4. Epub 2023 May 26.

Authors

Srdan Matosin^{1

2}, Patrick D Fischer^{1

2}, Maxim A Droemer^{1

2}, Eric Baggs³, Abu Sayeed Chowdhury⁴, Isidoro Tavares^{1

5

6}, Scott B Ficarro^{1

5

6}, Lisa Rose Warner³, Haribabu Arthanari^{1

2}, Rajesh Nagarajan⁷

Affiliations

¹ Department of Cancer Biology, Dana-Farber Cancer Institute, 02215, Boston, MA, USA.
² Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 02115, Boston, MA, USA.
³ Department of Chemistry and Biochemistry, Boise State University, Boise, ID, USA.
⁴ Biomolecular Sciences Graduate Program, Boise State University, Boise, ID, USA.
⁵ Blais Proteomics Center, Dana-Farber Cancer Institute, Boston, MA, USA.
⁶ Center for Emergent Drug Targets, Dana-Farber Cancer Institute, Boston, MA, USA.
⁷ Department of Chemistry and Biochemistry, Boise State University, Boise, ID, USA. [email protected].

Abstract

Acyl carrier proteins (ACPs) are universally conserved proteins amongst different species and are involved in fatty acid synthesis. Bacteria utilize ACPs as acyl carriers and donors for the synthesis of products such as endotoxins or acyl homoserine lactones (AHLs), which are used in quorum sensing mechanisms. In this study, wehave expressed isotopically labeled holo-ACP from Burkholderia mallei in Escherichia coli to assign 100% of non-proline backbone amide (HN) resonances, 95.5% of aliphatic carbon resonances and 98.6% of aliphatic hydrogen sidechain resonances.

Keywords: Acyl carrier proteins; NMR assignment; Quorum sensing.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acyl Carrier Protein* / metabolism
Bacterial Proteins / metabolism
Burkholderia mallei* / metabolism
Escherichia coli / metabolism
Nuclear Magnetic Resonance, Biomolecular

Substances

Acyl Carrier Protein
Bacterial Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding