Immunohistologic Features of Cerebral Venous Thrombosis Due to Vaccine-Induced Immune Thrombotic Thrombocytopenia

Neurol Neuroimmunol Neuroinflamm. 2023 May 26;10(4):e200127. doi: 10.1212/NXI.0000000000200127. Print 2023 Jul.

Abstract

Objectives: Vaccine-induced immune thrombotic thrombocytopenia (VITT), a recently described entity characterized by thrombosis at unusual locations such as cerebral venous sinus and splanchnic vein, has been rarely described after adenoviral-encoded COVID-19 vaccines. In this study, we report the immunohistological correlates in 3 fatal cases of cerebral venous thrombosis related to VITT analyzed at an academic medical center.

Methods: Detailed neuropathologic studies were performed in 3 cases of cerebral venous thrombosis related to VITT after adenoviral COVID-19 vaccination.

Results: Autopsy revealed extensive cerebral vein thrombosis in all 3 cases. Polarized thrombi were observed with a high density of neutrophils in the core and a low density in the tail. Endothelial cells adjacent to the thrombus were largely destroyed. Markers of neutrophil extracellular trap and complement activation were present at the border and within the cerebral vein thrombi. SARS-CoV-2 spike protein was detected within the thrombus and in the adjacent vessel wall.

Discussion: Data indicate that neutrophils and complement activation associated with antispike immunity triggered by the vaccine is probably involved in the disease process.

MeSH terms

  • COVID-19 Vaccines / adverse effects
  • COVID-19*
  • Endothelial Cells
  • Humans
  • SARS-CoV-2
  • Thrombocytopenia*
  • Thrombosis*
  • Vaccines*
  • Venous Thrombosis* / etiology

Substances

  • spike protein, SARS-CoV-2
  • COVID-19 Vaccines
  • Vaccines