Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis

Oleksandr Kirsanov; Christopher B Geyer

doi:10.1007/978-1-0716-3139-3_13

Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis

Methods Mol Biol. 2023:2656:227-237. doi: 10.1007/978-1-0716-3139-3_13.

Authors

Oleksandr Kirsanov¹, Christopher B Geyer^{2

3}

Affiliations

¹ Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
² Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC, USA. [email protected].
³ East Carolina Diabetes and Obesity Institute at East Carolina University, Greenville, NC, USA. [email protected].

Abstract

The delivery, to newborn and juvenile mice, of drugs and other compounds that manipulate the physiology or cellular/molecular state -e.g., by activating or inhibiting signaling pathways) is a powerful, yet underutilized approach to studying spermatogenesis. Here, we provide detailed protocols we have optimized in our laboratory for safely and effectively feeding and injecting mice and discuss troubleshooting approaches.

Keywords: Feeding; Injection; Newborn/neonatal; Spermatogenesis.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Male
Mice
Spermatogenesis*
Testis* / metabolism

Grants and funding

R01 HD090083/HD/NICHD NIH HHS/United States