Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa In-Field

Nicola Luchi; Duccio Migliorini; Francesco Pecori; Alberto Santini

doi:10.1007/978-1-0716-3159-1_4

Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa In-Field

Methods Mol Biol. 2023:2659:51-60. doi: 10.1007/978-1-0716-3159-1_4.

Authors

Nicola Luchi¹, Duccio Migliorini², Francesco Pecori², Alberto Santini²

Affiliations

¹ National Research Council, Institute for Sustainable Plant Protection (CNR-IPSP), Florence, Italy. [email protected].
² National Research Council, Institute for Sustainable Plant Protection (CNR-IPSP), Florence, Italy.

PMID: 37249884
DOI: 10.1007/978-1-0716-3159-1_4

Abstract

Early diagnosis is part of a decision-making process which in the case of plant diseases may prevent the spread of invasive plant pathogens and assist in their eradication. Significant advantages could be obtained from moving testing technology closer to the sampling site, thereby reducing the detection time. This chapter describes a portable real-time LAMP assay for a specific detection of Xylella fastidiosa in-field. The LAMP assay, including DNA extraction, allows a complete and specific in-field analysis in just 40 minutes, enabling the detection of pathogen DNA in host tissues.

Keywords: Bacterial pathogens; Detection; Invasive pathogen; Isothermal amplification; LAMP; Olive quick decline syndrome; Pierce’s disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Plant Diseases
Xylella* / genetics

Substances

DNA

Supplementary concepts

Xylella fastidiosa
LAMP assay