Antibacterial Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death Associated with Extracellular Trap Release

Tiziano A Schweizer; Sanne Hertegonne; Clément Vulin; Annelies S Zinkernagel; Srikanth Mairpady Shambat

doi:10.1007/978-1-0716-3243-7_16

Antibacterial Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death Associated with Extracellular Trap Release

Methods Mol Biol. 2023:2674:235-250. doi: 10.1007/978-1-0716-3243-7_16.

Authors

Tiziano A Schweizer¹, Sanne Hertegonne¹, Clément Vulin¹, Annelies S Zinkernagel¹, Srikanth Mairpady Shambat²

Affiliations

¹ Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich and University of Zurich, Zurich, Switzerland.
² Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich and University of Zurich, Zurich, Switzerland. [email protected].

PMID: 37258972
DOI: 10.1007/978-1-0716-3243-7_16

Abstract

Regulated cell death (RCD) and the concomitant release of extracellular traps by neutrophils (NETs) constitute an important antibacterial effector response. Usually, the dynamic processes of RCD and NETs release are assessed independently of each other by either unspecific or time-consuming methods. Here, we describe a flow cytometry-based high-throughput analysis method incorporating neutrophil RCD and NETs release with visual live-imaging conformation upon ex vivo bacterial challenge. This combined approach allows to quantify and closely follow the kinetics of the dynamic neutrophil effector response towards bacterial infection.

Keywords: Bacterial infection; Neutrophils; Neutrophil extracellular traps; Regulated cell death; Staphylococcus aureus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology
Extracellular Traps* / metabolism
Flow Cytometry / methods
Neutrophils / metabolism
Regulated Cell Death*

Substances

Anti-Bacterial Agents