Quantitative trait loci for resistance to black point caused by Bipolaris sorokiniana in bread wheat

Chuang Gao; Gaili Song; Kefei Qu; Mengyu Li; Yumei Jiang; Guihong Yin; Jishan Niu; Jianwei Tang; Yan Gao; Qiaoyun Li

doi:10.1007/s11032-023-01356-6

Quantitative trait loci for resistance to black point caused by Bipolaris sorokiniana in bread wheat

Mol Breed. 2023 Jan 28;43(2):10. doi: 10.1007/s11032-023-01356-6. eCollection 2023 Feb.

Authors

Chuang Gao¹, Gaili Song¹, Kefei Qu¹, Mengyu Li¹, Yumei Jiang¹, Guihong Yin¹, Jishan Niu¹, Jianwei Tang¹, Yan Gao¹, Qiaoyun Li¹

Affiliation

¹ National Engineering Research Centre for Wheat/College of Agronomy/Henan Technology Innovation Centre of Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, 15 Longzihu College District, Zhengzhou, 450046 Henan Province China.

Abstract

Black point disease is a serious concern in wheat production worldwide. In this study, we aimed to identify the major quantitative trait loci (QTL) for resistance to black point caused by Bipolaris sorokiniana and develop molecular markers for marker-assisted selection (MAS). A recombinant inbred line (RIL) population derived from a cross between PZSCL6 (highly susceptible) and Yuyou1 (moderately resistant) was evaluated for black point resistance at four locations under artificial inoculation with B. sorokiniana. Thirty resistant and 30 susceptible RILs were selected to form resistant and susceptible bulks, respectively, which were genotyped by the wheat 660 K SNP array. Two hundred and four single-nucleotide polymorphisms (SNPs) were identified, among which 41(20.7%), 34 (17.2%), 22 (11.1%), and 22 (11.1%) were located on chromosomes 5A, 5B, 4B, and 5D, respectively. The genetic linkage map for the RIL population was constructed using 150 polymorphic SSR and dCAPS markers. Finally, five QTL were detected on chromosomes 5A, 5B, and 5D, designated QBB.hau-5A, QBB.hau-5B.1, QBB.hau-5B.2, QBB.hau-5D.1, and QBB.hau-5D.2, respectively. All resistance alleles were contributed by the resistant parent Yuyou1. QBB.hau-5D.1 is likely to be a new locus for black point resistance. The markers Xwmc654 and Xgwm174 linked to QBB.hau-5A and QBB.hau-5D.1, respectively, have potential utility in MAS-based breeding.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-023-01356-6.

Keywords: Bipolaris sorokiniana; Black point disease; Marker-assisted selection; Quantitative trait locus; Triticum aestivum.

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