High fructan barley lines produced by selective breeding may alter β-glucan and amylopectin molecular structure

Shishanthi Jayarathna; Yunkai Jin; Gleb Dotsenko; Mingliang Fei; Mariette Andersson; Annica A M Andersson; Chuanxin Sun; Roger Andersson

doi:10.1016/j.carbpol.2023.121030

High fructan barley lines produced by selective breeding may alter β-glucan and amylopectin molecular structure

Carbohydr Polym. 2023 Sep 15:316:121030. doi: 10.1016/j.carbpol.2023.121030. Epub 2023 May 16.

Authors

Shishanthi Jayarathna¹, Yunkai Jin², Gleb Dotsenko³, Mingliang Fei⁴, Mariette Andersson⁵, Annica A M Andersson⁶, Chuanxin Sun⁷, Roger Andersson⁸

Affiliations

¹ Department of Molecular Sciences, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: [email protected].
² Department of Plant Biology, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden. Electronic address: [email protected].
³ Department of Molecular Sciences, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden.
⁴ Department of Plant Biology, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden; Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, Hunan Agricultural University, Changsha 410128, China; Key Laboratory of Education Department of Hunan Province on Plant Genetics and Molecular Biology, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
⁵ Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, SE-234 22 Lomma, Sweden. Electronic address: [email protected].
⁶ Department of Molecular Sciences, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: [email protected].
⁷ Department of Plant Biology, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden. Electronic address: [email protected].
⁸ Department of Molecular Sciences, BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: [email protected].

PMID: 37321727
DOI: 10.1016/j.carbpol.2023.121030

Abstract

Six cross-bred barley lines developed by a breeding strategy with the target to enhance the fructan synthesis activity and reduce the fructan hydrolysis activity were analyzed together with their parental lines, and a reference line (Gustav) to determine whether the breeding strategy also affected the content and molecular structure of amylopectin and β-glucan. The highest fructan and β-glucan content achieved in the novel barley lines was 8.6 % and 12 %, respectively (12.3-fold and 3.2-fold higher than in Gustav). The lines with low fructan synthesis activity had higher starch content, smaller building blocks in amylopectin, and smaller structural units of β-glucans than the lines with high-fructan synthesis activity. Correlation analysis confirmed that low starch content was associated with high amylose, fructan, and β-glucan content, and larger building blocks in amylopectin.

Keywords: Amylopectin; Building block structure; Fructan; Starch; β-Glucan.

MeSH terms

Amylopectin / chemistry
Amylose / chemistry
Hordeum* / chemistry
Molecular Structure
Selective Breeding
Starch / chemistry
beta-Glucans*

Substances

Amylopectin
beta-Glucans
Starch
Amylose