Introduction: Shigella bacteria cause shigellosis, a gastrointestinal infection most often acquired from contaminated food or water.
Methods: In this review, the general characteristics of Shigella bacteria are described, cases of laboratory-acquired infections (LAIs) are discussed, and evidence gaps in current biosafety practices are identified.
Results: LAIs are undoubtedly under-reported. Owing to the low infectious dose, rigorous biosafety level 2 practices are required to prevent LAIs resulting from sample manipulation or contact with infected surfaces.
Conclusions: It is recommended that, before laboratory work with Shigella, an evidence-based risk assessment be conducted. Particular emphasis should be placed on personal protective equipment, handwashing, and containment practices for procedures that generate aerosols or droplets.
Keywords: Shigella spp.; biosafety evidence; disinfection; inactivation; knowledge gap analysis; pathogen characteristics.
© Stuart D. Blacksell et al. 2023; Published by Mary Ann Liebert, Inc.