When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related to virus viability.
Keywords: Spain; cell culture; monkeypox virus; mpox; orthopoxvirus; real-time PCR; sexually transmitted infections; viruses.