The OsbHLH002/OsICE1-OSH1 module orchestrates secondary cell wall formation in rice

Cell Rep. 2023 Jul 25;42(7):112702. doi: 10.1016/j.celrep.2023.112702. Epub 2023 Jun 28.

Abstract

Transcriptional regulation of secondary cell wall (SCW) formation is strictly controlled by a complex network of transcription factors in vascular plants and has been shown to be mediated by a group of NAC master switches. In this study, we show that in a bHLH transcription factor, OsbHLH002/OsICE1, its loss-of-function mutant displays a lodging phenotype. Further results show that OsbHLH002 and Oryza sativa homeobox1 (OSH1) interact and share a set of common targets. In addition, the DELLA protein SLENDER RICE1, rice ortholog of KNOTTED ARABIDOPSIS THALIANA7, and OsNAC31 interact with OsbHLH002 and OSH1 and regulate their binding capacity on OsMYB61, a key regulatory factor in SCW development. Collectively, our results indicate OsbHLH002 and OSH1 as key regulators in SCW formation and shed light on molecular mechanisms of how active and repressive factors precisely orchestrate SCW synthesis in rice, which may provide a strategy for manipulating plant biomass production.

Keywords: CP: Molecular biology; CP: Plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / genetics
  • Arabidopsis* / metabolism
  • Cell Wall / metabolism
  • Gene Expression Regulation, Plant
  • Oryza* / genetics
  • Plant Proteins / metabolism
  • Transcription Factors / metabolism

Substances

  • Arabidopsis Proteins
  • Transcription Factors
  • Plant Proteins