Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

Xiao Liu; Kevin Lam; Huayi Zhao; Sadatsugu Sakane; Hyun Young Kim; Alvaro Eguileor; Karin Diggle; Shuai Wu; Raquel Carvalho Gontijo Weber; Pejman Soroosh; Mojgan Hosseini; Kristin Mekeel; David A Brenner; Tatiana Kisseleva

doi:10.1016/j.xpro.2023.102391

Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

STAR Protoc. 2023 Sep 15;4(3):102391. doi: 10.1016/j.xpro.2023.102391. Epub 2023 Jul 4.

Authors

Affiliations

¹ Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA. Electronic address: [email protected].
² Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
³ Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
⁴ State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China.
⁵ Janssen Pharmaceutical R&D, Immunometabolism Obesity and Metabolic Disorders, San Diego, CA, USA.
⁶ Department of Pathology, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
⁷ Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
⁸ Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA. Electronic address: [email protected].

Abstract

Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research.

Keywords: Cell Biology; Health Sciences.

MeSH terms

Cell Separation / methods
Cells, Cultured
Hepatocytes
Humans
Non-alcoholic Fatty Liver Disease*

Abstract

MeSH terms

Grants and funding