A protocol to investigate neural coupling of brain oscillations in rodents using in vivo electrophysiological recordings

Cong Wang; Peter G Stratton; Pankaj Sah; Roger Marek

doi:10.1016/j.xpro.2023.102414

A protocol to investigate neural coupling of brain oscillations in rodents using in vivo electrophysiological recordings

STAR Protoc. 2023 Sep 15;4(3):102414. doi: 10.1016/j.xpro.2023.102414. Epub 2023 Jul 11.

Authors

Cong Wang¹, Peter G Stratton², Pankaj Sah³, Roger Marek⁴

Affiliations

¹ Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai 201203, China; Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: [email protected].
² School of Electrical Engineering and Robotics, Queensland University of Technology, Brisbane, QLD 4000, Australia.
³ Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia; Joint Center for Neuroscience and Neural Engineering, and Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong Province 518055, P. R. China. Electronic address: [email protected].
⁴ Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: [email protected].

Abstract

Memory processes are highly dependent on a cross-talk between brain regions via synchronized neural oscillations. Here, we present a protocol to perform multi-site electrophysiological recordings in vivo in freely moving rodents to investigate functional connectivity across brain regions during memory processes. We describe steps for recording local field potentials (LFPs) during behavior, extracting LFP bands, and analyzing synchronized LFP activity across brain regions. This technique also provides the potential to simultaneously assess single unit activity using tetrodes. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.

Keywords: Biotechnology and bioengineering; Neuroscience.

MeSH terms

Animals
Brain* / physiology
Electrophysiological Phenomena
Rodentia*