The ABI3-ERF1 module mediates ABA-auxin crosstalk to regulate lateral root emergence

Cell Rep. 2023 Jul 25;42(7):112809. doi: 10.1016/j.celrep.2023.112809. Epub 2023 Jul 13.

Abstract

Abscisic acid (ABA) is involved in lateral root (LR) development, but how ABA signaling interacts with auxin signaling to regulate LR formation is not well understood. Here, we report that ABA-responsive ERF1 mediates the crosstalk between ABA and auxin signaling to regulate Arabidopsis LR emergence. ABI3 is a negative factor in LR emergence and transcriptionally activates ERF1 by binding to its promoter, and reciprocally, ERF1 activates ABI3, which forms a regulatory loop that enables rapid signal amplification. Notably, ABI3 physically interacts with ERF1, reducing the cis element-binding activities of both ERF1 and ABI3 and thus attenuating the expression of ERF1-/ABI3-regulated genes involved in LR emergence and ABA signaling, such as PIN1, AUX1, ARF7, and ABI5, which may provide a molecular rheostat to avoid overamplification of auxin and ABA signaling. Taken together, our findings identify the role of the ABI3-ERF1 module in mediating crosstalk between ABA and auxin signaling in LR emergence.

Keywords: ABA; ABI3; CP: Plants; ERF1; auxin; lateral root.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / metabolism
  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / metabolism
  • Gene Expression Regulation, Plant
  • Indoleacetic Acids / metabolism
  • Peptide Termination Factors* / genetics
  • Peptide Termination Factors* / metabolism
  • Transcription Factors* / genetics
  • Transcription Factors* / metabolism

Substances

  • ABI3 protein, Arabidopsis
  • Abscisic Acid
  • Arabidopsis Proteins
  • Indoleacetic Acids
  • Transcription Factors
  • eukaryotic release factor 1, Arabidopsis
  • Peptide Termination Factors