Monitoring the development of Ctenocephalides felis felis (Siphonaptera, Pulicidae) eggs from a laboratory colony in Brazil subjected to different adhesive substances

This study aimed to evaluate the use of different adhesive substances in an method to monitor the development of C. felis felis eggs. Three adhesive substances were selected: cyanoacrylate, stick glue, and liquid silicone. The eggs were obtained from a laboratory colony and placed on a thin layer of adhesive substance on the lid of a Petri dish to prevent contact with the larval growth substrate. One hundred eggs were used for each adhesive substance. The development of the eggs was monitored for three consecutive days, during which larval hatching and egg characteristics were observed. Of the 100 eggs incubated for each adhesive substance, 64, 51, and 76 larvae hatched and survived from the stick glue, cyanoacrylate, and liquid silicone plates, respectively. After 30 days of incubation, 59, 45, and 68 hatched adults were observed, respectively, in these groups. The mean hatching of larvae and adults between the liquid silicone and cyanoacrylate groups differed statistically (p < 0.05). This study demonstrated positive results, and that liquid silicone was the most easily applicable adhesive substance on the plate and interfered less with egg development, therefore having the best larval recovery compared to incubated eggs compared to the other substances.