The Oxysterol Receptor EBI2 Links Innate and Adaptive Immunity to Limit IFN Response and Systemic Lupus Erythematosus

Adv Sci (Weinh). 2023 Sep;10(27):e2207108. doi: 10.1002/advs.202207108. Epub 2023 Jul 19.

Abstract

Systemic lupus erythematosus (SLE) is a complex autoimmune disease with abnormal activation of the immune system. Recent attention is increasing about how aberrant lipid and cholesterol metabolism is linked together with type I interferon (IFN-I) signaling in the regulation of the pathogenesis of SLE. Here, a metabonomic analysis is performed and increased plasma concentrations of oxysterols, especially 7α, 25-dihydroxycholesterol (7α, 25-OHC), are identified in SLE patients. The authors find that 7α, 25-OHC binding to its receptor Epstein-Barr virus-induced gene 2 (EBI2) in macrophages can suppress STAT activation and the production of IFN-β, chemokines, and cytokines. Importantly, monocytes/macrophages from SLE patients and mice show significantly reduced EBI2 expression, which can be triggered by IFN-γ produced in activated T cells. Previous findings suggest that EBI2 enhances immune cell migration. Opposite to this effect, the authors demonstrate that EBI2-deficient macrophages produce higher levels of chemokines and cytokines, which recruits and activates myeloid cells,T and B lymphocytes to exacerbate tetramethylpentadecane-induced SLE. Together, via sensing the oxysterol 7α, 25-OHC, EBI2 in macrophages can modulate innate and adaptive immune responses, which may be used as a potential diagnostic marker and therapeutic target for SLE.

Keywords: Epstein-Barr virus-induced gene 2; interferon; macrophages; oxysterols; systemic lupus erythematosus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptive Immunity
  • Animals
  • Cytokines / metabolism
  • Epstein-Barr Virus Infections*
  • Herpesvirus 4, Human
  • Humans
  • Hydroxycholesterols / metabolism
  • Hydroxycholesterols / pharmacology
  • Lupus Erythematosus, Systemic*
  • Mice
  • Oxysterols*
  • Receptors, G-Protein-Coupled / genetics

Substances

  • Cytokines
  • Hydroxycholesterols
  • oxysterol binding protein
  • Oxysterols
  • Receptors, G-Protein-Coupled
  • GPR183 protein, human
  • Gpr183 protein, mouse