Neurological comorbidities and novel mutations in Turkish cases with neurofibromatosis type 1

Ideggyogy Sz. 2023 Jul 30;76(7-8):270-274. doi: 10.18071/isz.76.0270.

Abstract

Background and purpose: <p>Neuro&shy;fibromatosis type 1 (NF1) is a rare, auto&shy;somal dominant multisystemic disease. The NF1 gene is localized on chromosome 17q11.2. Patients with NF1 have different clinical presentations and comorbidities. The aim of the present study is to determine the novel mutations and neurological comorbidities of NF1.</p>.

Methods: <p>Patients who were diagnosed with NF1 by clinical criteria of the National Institutes of Health were included in the study. After a detailed examination, the NF1 gene was analysed with the help of next generation sequencing technology from pe&shy;ripheral blood samples via MiSeq (Illu&shy;mina, USA). Bioinformatic analyzes were per&shy;for&shy;med to evaluate the clinical sig&shy;ni&shy;fi&shy;cance of the detected variants via the in&shy;ternational databanks in accordance with the ACMG (American College of Medical Ge&shy;netics) guide&shy;line. In addition, cerebral-spinal MRI, cerebral angiography, and ENMG exa&shy;mi&shy;na&shy;tions were performed if deemed necessary.</p>.

Results: <p>Twenty patients (12 female, 8 male) were included in the study. The mean age was 25.8&plusmn;10 (10-56) years. Previously defined 13 different pathogenic mutations according to the ACMG criteria were identified in 18 patients. Also, two novel mutations were detected in 2 cases. Moreover, neurological comorbidities (moyamoya disease, multiple sclerosis, Charcot Marie Tooth Type 1A) were found in 3 patients with NF1.</p>.

Conclusion: <p>In the present study two novel mutations and three different neurological comorbidities were identified in NF1.</p>.

Background and purpose: <p>Az 1-es típus&uacute; neuro&shy;fib&shy;ro&shy;ma&shy;tosis (NF1) ritka, autoszom&aacute;lis domin&aacute;ns multisziszt&eacute;m&aacute;s betegs&eacute;g. Az NF1 g&eacute;n a 17q11.2 kromosz&oacute;m&aacute;n lokaliz&aacute;l&oacute;dik. NF1 ese&shy;t&eacute;n a betegek k&uuml;l&ouml;nb&ouml;ző klinikai megjelen&eacute;si form&aacute;kkal &eacute;s k&uuml;l&ouml;nb&ouml;ző t&aacute;rsbetegs&eacute;gekkel jelentkeznek. A jelen tanulm&aacute;ny c&eacute;lja az NF1 &uacute;j mut&aacute;ci&oacute;inak &eacute;s neurol&oacute;giai t&aacute;rsbetegs&eacute;geinek meghat&aacute;roz&aacute;sa.</p>.

Methods: <p>A vizsg&aacute;latba olyan betegeket vontunk be, akikn&eacute;l az amerikai Nemzeti Eg&eacute;sz&shy;s&eacute;g&uuml;gyi Int&eacute;zet (National Institutes of Health) klinikai krit&eacute;riumai alapj&aacute;n NF1-et diagnosztiz&aacute;ltak. A r&eacute;szletes vizsg&aacute;latot k&ouml;&shy;vetően az NF1 g&eacute;nt perif&eacute;ri&aacute;s v&eacute;rmint&aacute;kb&oacute;l &uacute;jgener&aacute;ci&oacute;s szekven&aacute;l&aacute;si technol&oacute;gia se&shy;gíts&eacute;g&eacute;vel elemezt&uuml;k MiSeq (Illumina, USA) haszn&aacute;lat&aacute;val. A kimutatott vari&aacute;nsok klinikai jelentős&eacute;g&eacute;nek &eacute;rt&eacute;kel&eacute;s&eacute;re bioinformatikai elemz&eacute;seket v&eacute;gezt&uuml;nk a nemzetk&ouml;zi adatb&aacute;zisokon kereszt&uuml;l, az ACMG (American Col&shy;lege of Medical Genetics) ir&aacute;nyelv&eacute;nek meg&shy;fe&shy;lelően. Ezenkív&uuml;l, sz&uuml;ks&eacute;g eset&eacute;n ce&shy;reb&shy;ralis-spinalis MR-, agyi angiogr&aacute;fi&aacute;s &eacute;s ENMG-vizsg&aacute;latokat v&eacute;gezt&uuml;nk.</p>.

Results: <p>H&uacute;sz beteg (12 nő, 8 f&eacute;rfi) ker&uuml;lt bevon&aacute;sra. Az &aacute;tlag&eacute;letkor 25,8 &plusmn; 10 (10&ndash;56) &eacute;v volt. Tizennyolc betegn&eacute;l az ACMG-krit&eacute;riumok szerint kor&aacute;bban m&aacute;r meg&shy;hat&aacute;rozott 13 k&uuml;l&ouml;nb&ouml;ző patog&eacute;n mu&shy;t&aacute;&shy;ci&oacute;t azonosítottunk. K&eacute;t &uacute;j mut&aacute;ci&oacute;t is ki&shy;mutattunk k&eacute;t esetben. Ezenkív&uuml;l, h&aacute;rom NF1-es betegn&eacute;l neurol&oacute;giai t&aacute;rsbetegs&eacute;geket (Moyamoya-k&oacute;r, sclerosis multiplex, Charcot&ndash;Marie&ndash;Tooth 1A típus) tal&aacute;ltunk.</p>.

Conclusion: <p>Jelen tanulm&aacute;ny k&eacute;t &uacute;j mut&aacute;ci&oacute;t &eacute;s h&aacute;rom k&uuml;l&ouml;nb&ouml;ző neurol&oacute;giai t&aacute;rsbetegs&eacute;get azonosított NF1-ben.</p>.

Keywords: CMT1A; moya-moya; multiple sclerosis; neurofibromatosis; novel mutations.

MeSH terms

  • Adolescent
  • Adult
  • Female
  • Humans
  • Male
  • Mutation
  • Neurofibromatosis 1* / diagnosis
  • Neurofibromatosis 1* / genetics
  • Young Adult

Substances

  • NF1 protein, human