Methionine sulfoxide reductases and cholesterol transporter STARD3 constitute an efficient system for detoxification of cholesterol hydroperoxides

J Biol Chem. 2023 Sep;299(9):105099. doi: 10.1016/j.jbc.2023.105099. Epub 2023 Jul 26.

Abstract

Methionine sulfoxide reductases (MSRs) are key enzymes in the cellular oxidative defense system. Reactive oxygen species oxidize methionine residues to methionine sulfoxide, and the methionine sulfoxide reductases catalyze their reduction back to methionine. We previously identified the cholesterol transport protein STARD3 as an in vivo binding partner of MSRA (methionine sulfoxide reductase A), an enzyme that reduces methionine-S-sulfoxide back to methionine. We hypothesized that STARD3 would also bind the cytotoxic cholesterol hydroperoxides and that its two methionine residues, Met307 and Met427, could be oxidized, thus detoxifying cholesterol hydroperoxide. We now show that in addition to binding MSRA, STARD3 binds all three MSRB (methionine sulfoxide reductase B), enzymes that reduce methionine-R-sulfoxide back to methionine. Using pure 5, 6, and 7 positional isomers of cholesterol hydroperoxide, we found that both Met307 and Met427 on STARD3 are oxidized by 6α-hydroperoxy-3β-hydroxycholest-4-ene (cholesterol-6α-hydroperoxide) and 7α-hydroperoxy-3β-hydroxycholest-5-ene (cholesterol-7α-hydroperoxide). MSRs reduce the methionine sulfoxide back to methionine, restoring the ability of STARD3 to bind cholesterol. Thus, the cyclic oxidation and reduction of methionine residues in STARD3 provides a catalytically efficient mechanism to detoxify cholesterol hydroperoxide during cholesterol transport, protecting membrane contact sites and the entire cell against the toxicity of cholesterol hydroperoxide.

Keywords: STARD3; cholesterol; cholesterol hydroperoxide; lipid peroxidation; methionine; methionine sulfoxide reductase; oxidation–reduction (redox); oxidative stress.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Cholesterol* / analogs & derivatives
  • Cholesterol* / metabolism
  • Endosomes / metabolism
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Hydrogen Peroxide* / metabolism
  • Lysosomes / metabolism
  • Membrane Proteins* / genetics
  • Membrane Proteins* / metabolism
  • Methionine / metabolism
  • Methionine Sulfoxide Reductases* / genetics
  • Methionine Sulfoxide Reductases* / metabolism
  • Oxidation-Reduction
  • Sulfoxides / metabolism

Substances

  • Cholesterol
  • cholesterol hydroperoxide
  • Hydrogen Peroxide
  • Methionine
  • methionine sulfoxide
  • Methionine Sulfoxide Reductases
  • Sulfoxides
  • STARD3 protein, human
  • Membrane Proteins