Five new species of Mysmenopsis from the Brazilian Amazonian region are described: M. rodriguesae n. sp. and M. nadineae n. sp. known by males and females, M snethlageae n. sp., M. lopardoae n. sp. and M. regiae n. sp. known only by males. Three species, M. cienaga Müller, 1987 (previously known from Peru and Colombia), M. penai Platnick & Shadab, 1978 (Ecuador and Colombia), and M. shushufindi Dupérré & Tapia, 2020 (Ecuador) are recorded for the first time in Brazilian Amazonian region. An additional record of M. penai from northeastern Brazil is included.