Complete sequences of conjugal helper plasmids pRK2013 and pEVS104

Ashley Hall; Timothy Donohue; Jason Peters

doi:10.17912/micropub.biology.000882

Complete sequences of conjugal helper plasmids pRK2013 and pEVS104

MicroPubl Biol. 2023 Jul 13:2023:10.17912/micropub.biology.000882. doi: 10.17912/micropub.biology.000882. eCollection 2023.

Authors

Ashley Hall^{1

2}, Timothy Donohue^{1

2

3}, Jason Peters^{1

2

3

4

5

6}

Affiliations

¹ Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States.
² Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin, United States.
³ Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, United States.
⁴ Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, United States.
⁵ Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin, United States.
⁶ Center for Genomic Science Integration, University of Wisconsin-Madison, Madison, Wisconsin, United States.

Abstract

We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to aid future engineering efforts.

Grants and funding

T32 GM007133/GM/NIGMS NIH HHS/United States