Characterization and determination of casein glycomacropeptide in dairy products by UHPLC-MS/MS based on its characteristic peptide

Qin Chen; Shiyun Lai; Lezhen Dong; Yahui Liu; Daodong Pan; Zhen Wu; Zufang Wu; Ying Zhou; Yiping Ren; Jingshun Zhang; Lingyi Liu; Lianliang Liu

doi:10.1016/j.foodchem.2023.137049

Characterization and determination of casein glycomacropeptide in dairy products by UHPLC-MS/MS based on its characteristic peptide

Food Chem. 2024 Jan 1:430:137049. doi: 10.1016/j.foodchem.2023.137049. Epub 2023 Aug 1.

Authors

Qin Chen¹, Shiyun Lai², Lezhen Dong¹, Yahui Liu¹, Daodong Pan¹, Zhen Wu¹, Zufang Wu¹, Ying Zhou³, Yiping Ren³, Jingshun Zhang⁴, Lingyi Liu⁵, Lianliang Liu⁶

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, School of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, PR China.
² Hangzhou Pupai Technology Co., Ltd, Hangzhou 310000, China.
³ Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
⁴ Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China. Electronic address: [email protected].
⁵ Department of Food Science and Technology, University of Nebraska-Lincoln, 68588 NE, USA.
⁶ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, School of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, PR China. Electronic address: [email protected].

PMID: 37544157
DOI: 10.1016/j.foodchem.2023.137049

Abstract

The ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS) method was built to quantify the casein glycomacropeptide (CGMP) in bovine dairy products accurately based on targeted proteomics. Qualitative analysis of theoretical peptides was carried out using high-resolution mass spectrometry (HRMS) and protein software. Isotope-labeled characteristic peptides were acquired via the labeled amino acid condensation method to correct the matrix effects. Peptide MAIPPK was the representative characteristic peptide for distinguishing the CGMP from κ-casein through trypsin digestion. After optimizing the pre-treatment conditions, the final 8% oxidant concentration was selected and the 10% formic acid concentration with 2.5 h oxidation time. Moreover, the results of methodological verification showed that the recovery rate was 103.7%, meanwhile the precision of inter-day and intra-day was less than 5%. In conclusion, the research demonstrated the characteristic peptide MAIPPK could quantitatively applied to detect CGMP in dairy products.

Keywords: Casein glycomacropeptide; Characteristic peptide; High-resolution mass spectrometry (HRMS); Targeted proteomics; Ultra-high-performance liquid chromatography (UHPLC).

MeSH terms

Animals
Caseins*
Cattle
Chromatography, High Pressure Liquid / methods
Dairy Products / analysis
Peptides / chemistry
Tandem Mass Spectrometry* / methods

Substances

caseinomacropeptide
Caseins
Peptides