Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

Jinzhi Duan; Yanan Sun; Juan D Matute; Richard S Blumberg

doi:10.1016/j.xpro.2023.102485

Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

STAR Protoc. 2023 Sep 15;4(3):102485. doi: 10.1016/j.xpro.2023.102485. Epub 2023 Aug 9.

Authors

Jinzhi Duan¹, Yanan Sun², Juan D Matute³, Richard S Blumberg⁴

Affiliations

¹ Division of Gastroenterology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: [email protected].
² Department of Medicine, Section of Hematology-Medical Oncology, Boston University School of Medicine, Boston Medical Center, Boston, MA 02118, USA.
³ Division of Gastroenterology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Division of Neonatology and Newborn Medicine, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
⁴ Division of Gastroenterology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: [email protected].

Abstract

Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4⁺ T cell differentiation in the intestine. We describe steps for isolating naïve CD4⁺ T cells from mouse spleens and transferring them to recipient mice. We detail procedures to isolate lamina propria cells and analyze CD4⁺ T subsets using flow cytometry. This protocol is useful in the study of mucosal immune functions. For complete details on the use and execution of this protocol, please refer to Duan et al.¹.

Keywords: Flow Cytometry/Mass Cytometry; Immunology.

MeSH terms

Animals
CD4-Positive T-Lymphocytes*
Cell Differentiation
Flow Cytometry
Intestine, Small
Mice
Mucous Membrane*

Abstract

MeSH terms

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