Safety and immunogenicity of a new formulation of a pentavalent DTwP-HepB-Hib vaccine in healthy Indian infants-A randomized study

PLoS One. 2023 Aug 15;18(8):e0284898. doi: 10.1371/journal.pone.0284898. eCollection 2023.

Abstract

Background: Pentavalent vaccines (DTP-HepB-Hib) have been introduced in many countries in their routine public immunization programmes to protect against diphtheria (D), tetanus (T), pertussis (P), hepatitis B (Hep B) and Hemophilus influenzae type b (Hib) diseases. This study compared the safety and immunogenicity of a new formulation of a whole-cell Bordetella pertussis (wP) based pentavalent vaccine (DTwP-HepB-Hib). The new formulation was developed using well-characterized hepatitis B and pertussis whole cell vaccine components.

Methods: This was a phase III, observer-blind, randomized, non-inferiority, multi-center study conducted in India among 460 infants who were followed up for safety and immunogenicity for 28 days after administration of three doses of either investigational or licensed comparator formulations at 6-8, 10-12 and 14-16 weeks of age.

Results: The investigational formulation of DTwP-HepB-Hib vaccine was non-inferior to the licensed formulation in terms of hepatitis B seroprotection rate (% of subjects with HepB antibodies ≥10mIU/mL were 99.1% versus 99.0%, respectively, corresponding to a difference of 0.1% (95% CI, -2.47 to 2.68)) and pertussis immune responses (adjusted geometric mean concentrations of antibodies for anti-PT were 76.7 EU/mL versus 63.3 EU/mL, with a ratio of aGMTs of 1.21 (95% CI, 0.89-1.64), and for anti-FIM were 1079 EU/mL versus 1129 EU/mL, with a ratio of aGMTs of 0.95 (95% CI, 0.73-1.24), respectively). The immune responses to other valences (D, T, and Hib) in the two formulations were also similar. The safety profile of both formulations was found to be similar and were well tolerated.

Conclusions: The investigational DTwP-HepB-Hib vaccine formulation was immunogenic and well-tolerated when administered as three dose primary series in infants.

Clinical trial registration: Clinical Trials Registry India number: CTRI/2018/12/016692.

Publication types

  • Clinical Trial, Phase III
  • Equivalence Trial
  • Multicenter Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diphtheria-Tetanus-Pertussis Vaccine* / adverse effects
  • Diphtheria-Tetanus-Pertussis Vaccine* / immunology
  • Drug Compounding
  • Female
  • Haemophilus Vaccines* / adverse effects
  • Haemophilus Vaccines* / immunology
  • Hepatitis B Vaccines* / adverse effects
  • Hepatitis B Vaccines* / immunology
  • Humans
  • India
  • Infant
  • Male

Substances

  • Diphtheria-Tetanus-Pertussis Vaccine
  • DtwP-HepB-Hib vaccine
  • Haemophilus Vaccines
  • Hepatitis B Vaccines

Grants and funding

This study was funded by Sanofi. Sanofi was responsible for the study design, clinical data management, analysis, decision to publish, and preparation of the manuscript.