Identification of type D Pasteurella multocida by counterimmunoelectrophoresis

J Clin Microbiol. 1986 Nov;24(5):721-3. doi: 10.1128/jcm.24.5.721-723.1986.

Abstract

A counterimmunoelectrophoresis (CIE) test was applied to serotype 35 isolates of type D Pasteurella multocida recovered from 32 cases of atrophic rhinitis (in swine) and 3 cases of snuffles (in rabbits). The CIE test was compared with the indirect hemagglutination (IHA) and acriflavine (AF) tests. Results of the CIE test correlated 100% with those of the IHA test whereas results of the AF test correlated 91.43% with those of the IHA test. The CIE test was rapid and simpler to perform compared with the IHA test and more sensitive than the AF test. Cross-reactions were not encountered with capsular antigens of P. multocida types A, B, and E in the CIE test. The CIE test was not found to be suitable for typing type A P. multocida strains.

Publication types

  • Comparative Study

MeSH terms

  • Acriflavine
  • Animals
  • Antigens, Bacterial / immunology
  • Counterimmunoelectrophoresis
  • Cross Reactions
  • Hemagglutination Tests
  • Humans
  • Pasteurella / classification*
  • Pasteurella / immunology
  • Serotyping

Substances

  • Antigens, Bacterial
  • Acriflavine