A novel paper-based electrochemiluminescence biosensor for non-destructive detection of pathogenic bacteria in real samples

The demand for sensitive, portable, and non-destructive analysis of pathogenic bacteria is of significance in point-of-care diagnosis. Herein, we constructed a smart electrochemiluminescence (ECL) biosensor by integrating a flexible paper-based sensing device and a disposable three-electrode detecting system. Staphylococcus aureus (S. aureus)-responsive cellulose paper was prepared by employing aptamer as recognition element and a probe DNA (probe DNA-GOD) tagged with glucose oxidase (GOD) as a signal amplification unit. The formation of aptamer-S. aureus complex mediated the quantitative release of probe DNA-GOD. The remaining probe DNA-GOD on the paper-based aptasensor was then activated by glucose, which resulted in a significant decrease in ECL signal. To further improve the ECL performance of biosensor, a large number of Ru(bpy)₃²⁺ molecules were embedded into porous zinc-based metal-organic frameworks (MOFs) to form Ru(bpy)₃²⁺ functionalized MOF nanoflowers (Ru-MOF-5 NFs). Such biosensor enabled accurate, non-destructive, and real-time monitoring of S. aureus-contaminated food samples, opening a new avenue for sensitive recognition of pathogenic bacteria.