The charge transport of 1,4,5,8-naphthalene diimide (NDI)-based molecules is explored. Experimental results show that the conductance of the TH-NDI molecular junction can be reversibly tuned by bias voltage and solvent, while the conductance of the PH-NDI junction is almost independent of the bias voltage and solvent. Based on these orthogonal stimuli, an AND logic gate of TH-NDI junction with an electric signal as the output is constructed. These results will advance the development of functional molecular devices.