To investigate the relationship between male semen parameters and sperm DNA fragment index with age. Adopt cross-sectional sampling survey design, 3 203 male patients who visited the Department of Reproductive Andrology in the Third Affiliated Hospital of Zhengzhou University from January 2019 to June 2021 were selected as subjects. Age range is 18-57 years, with the median age of 30 years. Through quartile regression analysis, the correlation between age and different male semen parameters and DNA fragment index (DFI) was presented. The study population was divided into ≤30 years old group and >30 years old group, and the correlation between age and semen volume, sperm concentration, total sperm count, progressive motility, total motility, percentage of normal sperm and DFI level were compared and analyzed. The results showed that there were significant differences in progressive motility, total motility and DFI level among different age groups (χ2=-4.608, -4.604, -7.719,P all <0.05), but there was no significant difference in semen volume, sperm concentration, total sperm count and percentage of normal sperm (χ2=-1.712, -1.203, -0.149, -0.175,P all >0.05). In the>30 years old age group, there was a very weak negative correlation between male age and semen volume, progressive motility and total motility (r=-0.137, -0.101 and -0.056, P all <0.05). There was a very weak positive correlation between male age and sperm concentration and sperm DFI level (r=0.061, 0.190, P all <0.05), while there was no correlation between male age and total sperm count and percentage of normal sperm (r=-0.018, -0.016,P all >0.05). In conclusion, with the increase of age, especially after the age of 30, semen volume, progressive motility and total motility decreased, while sperm concentration and DFI level increased, and semen quality decreased.
探讨男性精液参数和精子DNA碎片指数与年龄之间的关系。采用横断面抽样调查设计，选取2019年1月至2021年6月在郑州大学第三附属医院生殖男科就诊的3 203例男性患者作为研究对象，年龄范围18~57岁、中位年龄为30岁，通过分位数回归分析，呈现年龄与男性精液参数及DNA碎片指数（DFI）的相关关系。将研究人群分为≤30岁组和>30岁组，并分别比较两组的年龄与精液体积、精子浓度、精子总数、前向运动精子活力、总活力、正常形态精子百分率及DFI水平之间的相关性。结果显示，不同年龄组间前向运动精子活力、总活力及DFI水平差异有统计学意义（χ2=-4.608、-4.604、-7.719，P均<0.05）；精液体积、精子浓度、精子总数及正常形态精子百分率差异无统计学意义（χ2=-1.712、-1.203、-0.149、-0.175，P均>0.05）。>30岁年龄组中，男性年龄与精液体积、前向运动精子活力及总活力呈极弱负相关（r=-0.137、-0.101、-0.056，P均<0.05），男性年龄与精子浓度及精子DFI水平呈极弱正相关（r=0.061、0.190，P均<0.05），而男性年龄与精子总数及正常形态精子百分率无相关性（r=-0.018、-0.016，P均>0.05）。综上，随着年龄增长，特别是30岁以后，精液体积、前向运动精子活力及总活力降低，而精子浓度及DFI水平则升高，精液质量下降。.