Spatiotemporal observations of host-pathogen interactions in mucosa during SARS-CoV-2 infection indicate a protective role of ILC2s

Wei Hu; Lu Meng; Chao Wang; Wenhan Lu; Xiaoyu Tong; Rui Lin; Tao Xu; Liang Chen; An Cui; Xiaoqing Xu; Anni Li; Jia Tang; Hongru Gao; Zhenle Pei; Ruonan Zhang; Yicong Wang; Yu Wang; Wendong Han; Ning Jiang; Chenglong Xiong; Yi Feng; Kuinyu Lee; Mingquan Chen

doi:10.1128/spectrum.00878-23

Spatiotemporal observations of host-pathogen interactions in mucosa during SARS-CoV-2 infection indicate a protective role of ILC2s

Microbiol Spectr. 2023 Dec 12;11(6):e0087823. doi: 10.1128/spectrum.00878-23. Epub 2023 Nov 8.

Authors

Wei Hu^#^{1

2

3}, Lu Meng^#⁴, Chao Wang^#^{1

2}, Wenhan Lu^#³, Xiaoyu Tong³, Rui Lin⁵, Tao Xu⁶, Liang Chen⁷, An Cui^{1

2}, Xiaoqing Xu^{1

2}, Anni Li^{1

2}, Jia Tang^{1

2}, Hongru Gao³, Zhenle Pei³, Ruonan Zhang³, Yicong Wang³, Yu Wang³, Wendong Han⁸, Ning Jiang⁵, Chenglong Xiong⁹, Yi Feng³, Kuinyu Lee³, Mingquan Chen^{1

2}

Affiliations

¹ Department of Emergency Medicine, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University , Shanghai, China.
² Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University , Shanghai, China.
³ Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University , Shanghai, China.
⁴ The Center for Microbes, Development and Health, Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences , Shanghai, China.
⁵ State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University , Shanghai, China.
⁶ Department of Infectious Diseases, National Medical Center for Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Huashan Hospital, Shanghai Medical College, Fudan University , Shanghai, China.
⁷ State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing, China.
⁸ Biosafety Level 3 Laboratory, Shanghai Medical College Fudan University , Shanghai, China.
⁹ Department of Epidemiology, School of Public Health, and Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University , Shanghai, China.

^# Contributed equally.

Abstract

Our study revealed the spatial interaction between humanized ACE2 and pseudovirus expressing Spike, emphasizing the role of type 2 innate lymphoid cells during the initial phase of viral infection. These findings provide a foundation for the development of mucosal vaccines and other treatment approaches for both pre- and post-infection management of coronavirus disease 2019.

Keywords: ILC2; SARS-CoV-2; mucosal immunity; tissue transparency and 3D imaging.

MeSH terms

COVID-19*
Host-Pathogen Interactions
Humans
Immunity, Innate
Lymphocytes
Protein Binding
SARS-CoV-2

Abstract

MeSH terms

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