Objectives: To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood.
Methods: Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode.
Results: The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect.
Conclusions: This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine.
目的: 建立一种简便快速的血液中右美托咪定定性定量检测方法。方法: 血液经乙腈沉淀蛋白并过滤后，在Allure PFP Propyl液相色谱柱上分离，等度洗脱。采用正离子模式、多反应监测模式对右美托咪定进行定性定量分析。结果: 血液中右美托咪定的检出限为0.2 ng/mL，定量限为0.5 ng/mL，在0.5~1 000 ng/mL范围内线性关系良好，相关系数大于0.99。该方法准确度为90.34%~112.67%，提取回收率为50.05%~91.08%，无明显的基质效应。结论: 该方法样品前处理简单、选择性好，适用于血液中右美托咪定的定性定量分析，能够为涉右美托咪定的药物辅助犯罪案件提供借鉴和参考。.
Keywords: blood; dexmedetomidine; drug facilitated crimes; forensic toxicology; liquid chromatography-tandem mass spectrometry.