We developed an optical time-of-flight measurement system using a time-resolved and mode-selective up-conversion single-photon detector for acquiring tomographic images of a mouse brain. The probe and pump pulses were spectrally carved from a 100-femtosecond mode-locked fiber laser at 1556 nm using 4f systems, so that their center wavelengths were situated at either side of the phase matching band separated by 30 nm. We demonstrated a sensitivity of 111 dB which is comparable to that of shot-noise-limited optical coherence tomography and an axial resolution of 57 μm (a refractive index of 1.37) with 380 femtosecond probe and pump pulses whose average powers were 1.5 mW and 30 μW, respectively. The proposed technique will open a new way of non-contact and non-invasive three-dimensional structural imaging of biological specimens with ultraweak optical irradiation.
© 2023. The Author(s).