Structural characterization of M8C10, a neutralizing antibody targeting a highly conserved prefusion-specific epitope on the metapneumovirus fusion trimerization interface

J Virol. 2023 Dec 21;97(12):e0105223. doi: 10.1128/jvi.01052-23. Epub 2023 Nov 30.

Abstract

Human metapneumovirus (hMPV) is a common pathogen causing lower respiratory tract infections worldwide and can develop severe symptoms in high-risk populations such as infants, the elderly, and immunocompromised patients. There are no approved hMPV vaccines or neutralizing antibodies available for therapeutic or prophylactic use. The trimeric hMPV fusion F protein is the major target of neutralizing antibodies in human sera. Understanding the immune recognition of antibodies to hMPV-F antigen will provide critical insights into developing efficacious hMPV monoclonal antibodies and vaccines.

Keywords: B cell cloning; fusion protein; hMPV; metapneumovirus; neutralizing antibody.

MeSH terms

  • Aged
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Epitopes
  • Humans
  • Metapneumovirus* / physiology
  • Paramyxoviridae Infections* / immunology
  • Viral Fusion Proteins
  • Viral Vaccines / immunology

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Epitopes
  • Viral Fusion Proteins
  • Viral Vaccines

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