Drug Screening of 49 Compounds by Liquid Chromatography-Tandem Mass Spectrometry in Saliva

Xu Li; Natalie Plotkina; Janetta Bryksin

doi:10.1007/978-1-0716-3541-4_19

Drug Screening of 49 Compounds by Liquid Chromatography-Tandem Mass Spectrometry in Saliva

Methods Mol Biol. 2024:2737:195-214. doi: 10.1007/978-1-0716-3541-4_19.

Authors

Xu Li¹, Natalie Plotkina², Janetta Bryksin³

Affiliations

¹ Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.
² Clarity Clinical Laboratory, Valdosta, GA, USA.
³ Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA. [email protected].

PMID: 38036823
DOI: 10.1007/978-1-0716-3541-4_19

Abstract

In recent years, the use of oral fluid as a testing matrix for drug analysis has become increasingly popular due to its benefits, such as a possibility for direct observation of a sample collection and, thus, a lower risk of adulteration. To address this trend, we have developed a quantitative LC-MS/MS method that can simultaneously analyze 49 commonly prescribed compounds in oral fluid. The assay is performed on an AB Sciex 4500 electrospray ionization mass spectrometer in multiple reaction monitoring mode.

Keywords: Illicit drugs; LC-MS/MS; Liquid chromatography; Mass spectrometry; Oral fluid; Saliva; Therapeutic drug monitoring.

MeSH terms

Chromatography, Liquid
Drug Contamination
Drug Evaluation, Preclinical
Saliva* / chemistry
Spectrometry, Mass, Electrospray Ionization / methods
Tandem Mass Spectrometry* / methods