Cold atmospheric plasma sensitizes melanoma cells to targeted therapy agents in vitro

J Biophotonics. 2024 Mar;17(3):e202300356. doi: 10.1002/jbio.202300356. Epub 2023 Dec 19.

Abstract

Cold atmospheric plasma (CAP) has been reported to kill melanoma cells in vitro and in vivo. BRAF and MEK inhibitors are targeted therapy agents for advanced melanoma patients with BRAF mutations. However, low overall survival and relapse-free survival are still tough challenges due to drug resistance. In this study, we confirmed that CAP alleviated innate drug resistance and promoted the anti-tumor effect of targeted therapy in A875 and WM115 melanoma cells in vitro. Further, we revealed that CAP altered the expression of various molecules concerning MAPK and PI3K-AKT pathways in A875 cells. This study demonstrates that CAP promises to work as adjuvant treatment with targeted therapy to overcome drug resistance for malignant tumors in future.

Keywords: cold atmospheric plasma; drug resistance; melanoma; targeted therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents* / pharmacology
  • Cell Line, Tumor
  • Drug Resistance, Neoplasm
  • Humans
  • Melanoma* / drug therapy
  • Melanoma* / pathology
  • Mutation
  • Phosphatidylinositol 3-Kinases / genetics
  • Phosphatidylinositol 3-Kinases / pharmacology
  • Phosphatidylinositol 3-Kinases / therapeutic use
  • Plasma Gases* / pharmacology
  • Proto-Oncogene Proteins B-raf / genetics
  • Proto-Oncogene Proteins B-raf / metabolism
  • Proto-Oncogene Proteins B-raf / therapeutic use

Substances

  • Phosphatidylinositol 3-Kinases
  • Plasma Gases
  • Proto-Oncogene Proteins B-raf
  • Antineoplastic Agents