Vitamin B12-loaded chitosan-based nanoparticle-embedded polymeric nanofibers for sublingual and transdermal applications: Two alternative application routes for vitamin B12

Ece Guler; Humeyra Betul Yekeler; Gita Parviz; Saliha Aydin; Asima Asghar; Murat Dogan; Fakhera Ikram; Deepak M Kalaskar; Muhammet Emin Cam

doi:10.1016/j.ijbiomac.2023.128635

Vitamin B₁₂-loaded chitosan-based nanoparticle-embedded polymeric nanofibers for sublingual and transdermal applications: Two alternative application routes for vitamin B₁₂

Int J Biol Macromol. 2024 Feb;258(Pt 2):128635. doi: 10.1016/j.ijbiomac.2023.128635. Epub 2023 Dec 6.

Authors

Ece Guler¹, Humeyra Betul Yekeler², Gita Parviz³, Saliha Aydin⁴, Asima Asghar⁵, Murat Dogan⁶, Fakhera Ikram⁷, Deepak M Kalaskar⁸, Muhammet Emin Cam⁹

Affiliations

¹ Department of Pharmacology, Faculty of Pharmacy, İstanbul Kent University, İstanbul 34406, Türkiye; Department of Pharmacology, Faculty of Pharmacy, Marmara University, İstanbul 34854, Türkiye; Center for Nanotechnology and Biomaterials Application and Research, Marmara University, İstanbul 34722, Türkiye; UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK; MecNano Technologies, Cube Incibation, Teknopark İstanbul, İstanbul 34906, Türkiye.
² Department of Pharmacology, Faculty of Pharmacy, Marmara University, İstanbul 34854, Türkiye; Center for Nanotechnology and Biomaterials Application and Research, Marmara University, İstanbul 34722, Türkiye; UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK; MecNano Technologies, Cube Incibation, Teknopark İstanbul, İstanbul 34906, Türkiye.
³ Department of Pharmacology, Faculty of Pharmacy, Marmara University, İstanbul 34854, Türkiye; Center for Nanotechnology and Biomaterials Application and Research, Marmara University, İstanbul 34722, Türkiye; MecNano Technologies, Cube Incibation, Teknopark İstanbul, İstanbul 34906, Türkiye.
⁴ Department of Pharmacology, Faculty of Pharmacy, Marmara University, İstanbul 34854, Türkiye.
⁵ Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Pakistan.
⁶ Sivas Cumhuriyet University, Pharmacy Faculty, Pharmaceutical Biotechnology Department, Sivas, Türkiye.
⁷ Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Pakistan. Electronic address: [email protected].
⁸ UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK.
⁹ Department of Pharmacology, Faculty of Pharmacy, İstanbul Kent University, İstanbul 34406, Türkiye; Department of Pharmacology, Faculty of Pharmacy, Marmara University, İstanbul 34854, Türkiye; Center for Nanotechnology and Biomaterials Application and Research, Marmara University, İstanbul 34722, Türkiye; UCL Division of Surgery and Interventional Sciences, Rowland Hill Street, NW3 2PF London, UK; MecNano Technologies, Cube Incibation, Teknopark İstanbul, İstanbul 34906, Türkiye; Biomedical Engineering Department, University of Aveiro, 3810-193 Aveiro, Portugal; Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, İstanbul 34854, Türkiye; SFA R&D Laboratories, Teknopark İstanbul, İstanbul 34906, Türkiye; ATA BIO Technology, Teknopol İstanbul, İstanbul 34930, Türkiye. Electronic address: [email protected].

PMID: 38065445
DOI: 10.1016/j.ijbiomac.2023.128635

Abstract

Alzheimer's disease (AD) is a neurodegeneration type that is biologically recognizable via β-amyloid plaques and tau neurofibril tangles. Global estimation for the total count of individuals enduring AD will rise up to 131 million by 2050. Investigations suggested the existence of a direct proportion between the likelihood of AD occurrence and vitamin B₁₂ (VB₁₂) hypovitaminosis. Approved VB₁₂ administrations, intramuscular and oral, each has serious defects broaching the demand for alternative routes. This work developed VB₁₂-loaded chitosan/tripolyphosphate/polyvinyl alcohol (CS/TPP/PVA) nanoparticles (NPs) embedded in polyvinylpyrrolidone (PVP) and polyvinylpyrrolidone/polycaprolactone (PVP/PCL) nanofibrous (NFs) produced by pressurized gyration (PG) for sublingual and transdermal routes, respectively. Biomaterials were investigated morphologically, chemically, and thermally. Moreover, degradation, disintegration, release behavior, and release kinetics were analyzed. The effectiveness and safety of nanomaterials were assessed and proven with the alamarBlue test on the Aβ_1-42-induced SH-SY5Y model. The final evaluation suggested the feasibility, safety, and effectiveness of produced systems. Consequently, two alternative VB₁₂ application routes were developed with high effectivity and low toxicity with the power of nanotechnology.

Keywords: Alzheimer's disease; Drug delivery; Vitamin B(12).

MeSH terms

Chitosan*
Humans
Nanofibers*
Nanoparticles*
Neuroblastoma*
Povidone
Vitamin B 12
Vitamins

Substances

Vitamin B 12
Chitosan
Povidone
Vitamins