In situ bioprinting of double network anti-digestive xanthan gum derived hydrogel scaffolds for the treatment of enterocutaneous fistulas

Kang Chen; Jinjian Huang; Ze Li; Jinpeng Zhang; Sicheng Li; Canwen Chen; Ye Liu; Guiwen Qu; Yitian Teng; Rui Ma; Zongan Li; Yungang Jiang; Kanglei Wang; Jun Chen; Xiuwen Wu; Jianan Ren

doi:10.1016/j.carbpol.2023.121508

In situ bioprinting of double network anti-digestive xanthan gum derived hydrogel scaffolds for the treatment of enterocutaneous fistulas

Carbohydr Polym. 2024 Feb 15:326:121508. doi: 10.1016/j.carbpol.2023.121508. Epub 2023 Oct 31.

Authors

Kang Chen¹, Jinjian Huang¹, Ze Li¹, Jinpeng Zhang¹, Sicheng Li¹, Canwen Chen¹, Ye Liu², Guiwen Qu², Yitian Teng¹, Rui Ma², Zongan Li³, Yungang Jiang¹, Kanglei Wang¹, Jun Chen⁴, Xiuwen Wu⁵, Jianan Ren⁶

Affiliations

¹ Research Institute of General Surgery, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.
² School of Medicine, Southeast University, Nanjing 210009, China.
³ Jiangsu Key Laboratory of 3D Printing Equipment and Manufacturing, NARI School of Electrical and Automation Engineering, Nanjing Normal University, Nanjing 210042, China.
⁴ Research Institute of General Surgery, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China. Electronic address: [email protected].
⁵ Research Institute of General Surgery, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China; School of Medicine, Southeast University, Nanjing 210009, China. Electronic address: [email protected].
⁶ Research Institute of General Surgery, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China; School of Medicine, Southeast University, Nanjing 210009, China. Electronic address: [email protected].

PMID: 38142061
DOI: 10.1016/j.carbpol.2023.121508

Abstract

The clinical treatment of enterocutaneous fistula is challenging and causes significant patient discomfort. Fibrin gel can be used to seal tubular enterocutaneous fistulas, but it has low strength and poor digestion resistance. Based on in situ bioprinting and the anti-digestive properties of xanthan gum (XG), we used carboxymethyl chitosan (CMC) and xanthan gum modified by grafted glycidyl methacrylate (GMA) and aldehyde (GCX) as the ink to print a double network hydrogel that exhibited high strength and an excellent anti-digestive performance. In addition, in vitro studies confirmed the biocompatibility, degradability, and self-healing of hydrogels. In our rabbit tubular enterocutaneous fistula model, the in situ printed hydrogel resisted corrosion due to the intestinal fluid and acted as a scaffold for intestinal mucosal cells to proliferate on its surface. To summarize, in situ bioprinting GCX/CMC double network hydrogel can effectively block tubular enterocutaneous fistulas and provide a stable scaffold for intestinal mucosal regeneration.

Keywords: Anti-digestive; Bioprinting; Carboxymethyl chitosan; Double network hydrogel; Enterocutaneous fistula; Intestinal fistula.

MeSH terms

Animals
Bioprinting*
Humans
Hydrogels
Intestinal Fistula*
Polysaccharides, Bacterial / therapeutic use
Rabbits

Substances

Hydrogels
xanthan gum
Polysaccharides, Bacterial