We report the complete genome and the plasmid (F' episome) sequences of Escherichia coli JM101 assembled with a combination of Nanopore and Illumina data. The resulting genome is a single contig of 4,524,963 bp, and the plasmid consists of a single contig of 197,186 bp.
Keywords: Escherichia coli JM101; F′ episome; Illumina; Nanopore; complete genome.