Modular coupling MOF nanozyme with natural enzyme on hollow fiber membrane for rapid and reusable detection of H2O2 and glucose

Mikrochim Acta. 2024 Jan 19;191(2):107. doi: 10.1007/s00604-023-06150-x.

Abstract

A novel strategy based on gradient porous hollow fiber membrane (GPF) is proposed for the modular assembly of enzyme-nanozyme cascade systems. The porous structure of GPF provided sufficient specific surface area, while the gradient structure effectively minimized the leaching of enzymes and nanozymes. To enhance stability, we prepared and immobilized metal-organic framework (MOF) nanozymes, resulting in the fabrication of GPF-MOF with excellent stability and reusability for colorimetric H2O2 detection. To improve specificity and expand the detection range, micro-crosslinked natural enzymes were modularly assembled, using glucose oxidase as the model enzyme. The assembled system, GPF-mGOx@MOF, achieved a low detection limit of 0.009 mM and a linear range of 0.2 to 11 mM. The sensor retained 87.2% and 80.7% of initial activity after being stored for 49 days and 9 recycles, respectively. Additionally, the reliability of the biosensor was validated through glucose determination of human blood and urine samples, yielding comparable results to a commercial glucose meter.

Keywords: Cascade colorimetric sensor; Gradient porous hollow fiber membrane; Rapid detection; Reusability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Glucose / chemistry
  • Glucose Oxidase / chemistry
  • Humans
  • Hydrogen Peroxide / chemistry
  • Metal-Organic Frameworks* / chemistry
  • Reproducibility of Results

Substances

  • Metal-Organic Frameworks
  • Glucose
  • Hydrogen Peroxide
  • Glucose Oxidase