Generation of a human induced pluripotent stem cell line (UMGACBi001-A) from urine cells of a chronic kidney disease patient with hypertension, diabetic nephropathy and acute sepsis

Stem Cell Res. 2023 Dec:73:103224. doi: 10.1016/j.scr.2023.103224. Epub 2023 Oct 20.

Abstract

Chronic kidney disease is a major public health burden associated with a drastically reduced quality of living and life span that lacks suitable, individualized therapeutic strategies. Here we present a human induced pluripotent stem cell line (iPSC, UMGACBi001-A) reprogrammed from urine cells of an acute septic dialysis patient suffering from chronic kidney disease using non-integrating administration of RNAs. The generated iPSCs were positively characterized for typical morphology, pluripotency marker expression, directed differentiation potential, non-contamination, chromosomal consistency and donor identity. This iPSC-line can be a useful source for in vitro disease modelling and individualized therapeutic approaches.

MeSH terms

  • Cell Differentiation
  • Diabetes Mellitus* / metabolism
  • Diabetic Nephropathies* / metabolism
  • Humans
  • Hypertension* / metabolism
  • Induced Pluripotent Stem Cells* / metabolism
  • Renal Insufficiency, Chronic* / metabolism
  • Sepsis* / metabolism