Premenstrual syndrome (PMS) has been known to have emotional, physical, and behavioral effects in reproductive age. To evaluate the effects of premenstrual syndrome (PMS) on pain and quality of life in women, who perform regular yoga exercises in the long term. 30 women with PMS and 30 control (31.17 ± 5.28 years old) participated in this randomized controlled, single blinded study. The demographics of the PMS and control groups were similar (p < 0.05). A significant difference was found in a depressive mood, anxiety, fatigue, pain, depressive thoughts, sleeping habits subscales, and total scores of the PMSS, WHOQOL-BREF scale, and VAS scores between yoga and control groups (p < 0.05). It is determined that long-term and regular yoga practice for women with PMS will improve their, PMS symptoms, quality of life, and pain level.