A Hitchhiker's guide to CRISPR editing tools in bacteria : CRISPR can help unlock the bacterial world, but technical and regulatory barriers persist

Nicolas Krink; Pablo Iván Nikel; Chase L Beisel

doi:10.1038/s44319-024-00086-w

A Hitchhiker's guide to CRISPR editing tools in bacteria : CRISPR can help unlock the bacterial world, but technical and regulatory barriers persist

EMBO Rep. 2024 Apr;25(4):1694-1699. doi: 10.1038/s44319-024-00086-w. Epub 2024 Feb 12.

Authors

Nicolas Krink¹, Pablo Iván Nikel¹, Chase L Beisel^{2

3}

Affiliations

¹ The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800, Kongens, Lyngby, Denmark.
² Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 97080, Würzburg, Germany. [email protected].
³ Medical Faculty, University of Würzburg, 97080, Würzburg, Germany. [email protected].

Abstract

Join us on a journey through the complex and ever-expanding universe of CRISPR approaches for genome editing in bacteria. Discover what is available, current technical challenges, successful implementation of these tools and the regulatory framework around their use.

MeSH terms

Bacteria* / genetics
CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats* / genetics
Gene Editing

Abstract

MeSH terms

Grants and funding