Characterization of an outbreak caused by Elizabethkingia miricola using Fourier-transform infrared (FTIR) spectroscopy

Eur J Clin Microbiol Infect Dis. 2024 Apr;43(4):797-803. doi: 10.1007/s10096-024-04764-4. Epub 2024 Feb 14.

Abstract

Fourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused by Elizabethkingia miricola. During the 2020-2021 period, 26 isolates (23 clinical and 3 environmental) were collected and analyzed by FTIR (IR Biotyper) and core-genome MLST (cgMLST), in addition to antimicrobial susceptibility testing. FTIR spectroscopy and cgMLST showed that 22 of the isolates were related to the outbreak, including the environmental samples, with only one discordance between both methods. Then, FTIR is useful for E. miricola typing and can be easily implemented in the laboratory.

Keywords: Elizabethkingia miricola; Bacterial typing; Fourier-transform infrared spectroscopy; Nosocomial outbreak.

MeSH terms

  • Disease Outbreaks
  • Flavobacteriaceae* / genetics
  • Humans
  • Multilocus Sequence Typing
  • Spectroscopy, Fourier Transform Infrared

Supplementary concepts

  • Elizabethkingia miricola