Clinical isolates of Streptococcus mitis/oralis-related species with reduced carbapenem susceptibility, harboring amino acid substitutions in penicillin-binding proteins in Japan

Antimicrob Agents Chemother. 2024 Apr 3;68(4):e0117923. doi: 10.1128/aac.01179-23. Epub 2024 Feb 28.

Abstract

Streptococcus mitis/oralis group isolates with reduced carbapenem susceptibility have been reported, but its isolation rate in Japan is unknown. We collected 356 clinical α-hemolytic streptococcal isolates and identified 142 of them as S. mitis/oralis using partial sodA sequencing. The rate of meropenem non-susceptibility was 17.6% (25/142). All 25 carbapenem-non-susceptible isolates harbored amino acid substitutions in/near the conserved motifs in PBP1A, PBP2B, and PBP2X. Carbapenem non-susceptibility is common among S. mitis/oralis group isolates in Japan.

Keywords: Streptococcus mitis/oralis; penicillin-binding protein; reduced carbapenem susceptibility.

MeSH terms

  • Amino Acid Substitution
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Carbapenems* / pharmacology
  • Japan
  • Microbial Sensitivity Tests
  • Penicillin-Binding Proteins / genetics
  • Streptococcus / metabolism
  • Streptococcus mitis* / genetics
  • Streptococcus mitis* / metabolism
  • Viridans Streptococci / genetics

Substances

  • Penicillin-Binding Proteins
  • Carbapenems
  • Bacterial Proteins