Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins

Fu Kuroiwa; Hiraku Suda; Maho Yabuki; Kimie Atsuzawa; Haruhiko Yamaguchi; Masatsugu Toyota; Yasuko Kaneko; Satoshi Yamashita; Seiji Takahashi; Yuzuru Tozawa

doi:10.1093/bbb/zbae026

Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins

Biosci Biotechnol Biochem. 2024 Apr 22;88(5):555-560. doi: 10.1093/bbb/zbae026.

Authors

Affiliations

¹ Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
² Comprehensive Analysis Center for Science, Saitama University, Saitama, Japan.
³ Sumitomo Rubber Industries Ltd., Kobe, Hyogo, Japan.
⁴ Department of Natural Science in the Faculty of Education, Saitama University, Saitama, Japan.
⁵ Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Japan.
⁶ Graduate School of Engineering, Tohoku University, Sendai, Miyagi, Japan.

PMID: 38444196
DOI: 10.1093/bbb/zbae026

Abstract

Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm the properties of proteins that specifically bind to lipid monolayers in a translation-coupled manner.

Keywords: in vitro protein synthesis; cell-free translation; lipid monolayer; membrane protein.

MeSH terms

Cell-Free System*
Lipids* / chemistry
Protein Binding
Protein Biosynthesis*
Proteins / chemistry
Proteins / metabolism

Substances

Lipids
Proteins

Grants and funding

JP23KJ0297/Japan Society for the Promotion of Science