Engagement of sialylated glycans with Siglec receptors on suppressive myeloid cells inhibits anticancer immunity via CCL2

Cell Mol Immunol. 2024 May;21(5):495-509. doi: 10.1038/s41423-024-01142-0. Epub 2024 Mar 6.

Abstract

The overexpression of sialic acids on glycans, called hypersialylation, is a common alteration found in cancer cells. Sialylated glycans can enhance immune evasion by interacting with sialic acid-binding immunoglobulin-like lectin (Siglec) receptors on tumor-infiltrating immune cells. Here, we investigated the effect of sialylated glycans and their interaction with Siglec receptors on myeloid-derived suppressor cells (MDSCs). We found that MDSCs derived from the blood of lung cancer patients and tumor-bearing mice strongly express inhibitory Siglec receptors and are highly sialylated. In murine cancer models of emergency myelopoiesis, Siglec-E knockout in myeloid cells resulted in prolonged survival and increased tumor infiltration of activated T cells. Targeting suppressive myeloid cells by blocking Siglec receptors or desialylation strongly reduced their suppressive potential. We further identified CCL2 as a mediator involved in T-cell suppression upon interaction between sialoglycans and Siglec receptors on MDSCs. Our results demonstrated that sialylated glycans inhibit anticancer immunity by modulating CCL2 expression.

Keywords: Sialic acid-binding immunoglobulin-like lectin; myeloid derived suppressor cells; sialoglycans; tumor microenvironment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Chemokine CCL2* / metabolism
  • Humans
  • Lung Neoplasms / immunology
  • Lung Neoplasms / pathology
  • Mice
  • Mice, Inbred C57BL*
  • Mice, Knockout
  • Myeloid-Derived Suppressor Cells* / immunology
  • Myeloid-Derived Suppressor Cells* / metabolism
  • N-Acetylneuraminic Acid / metabolism
  • Polysaccharides* / metabolism
  • Sialic Acid Binding Immunoglobulin-like Lectins* / metabolism
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism

Substances

  • Sialic Acid Binding Immunoglobulin-like Lectins
  • Polysaccharides
  • Chemokine CCL2
  • N-Acetylneuraminic Acid