Genomic diversity of group A rotaviruses from wild boars and domestic pigs in Japan: wide prevalence of NSP5 carrying the H2 genotype

Shigeki Shizawa; Fujiko Fukuda; Yasuhiro Kikkawa; Toru Oi; Hitoshi Takemae; Tsuneyuki Masuda; Hiroho Ishida; Hironobu Murakami; Shoichi Sakaguchi; Tetsuya Mizutani; Makoto Nagai; Mami Oba

doi:10.1007/s00705-023-05954-9

Genomic diversity of group A rotaviruses from wild boars and domestic pigs in Japan: wide prevalence of NSP5 carrying the H2 genotype

Arch Virol. 2024 Mar 7;169(3):63. doi: 10.1007/s00705-023-05954-9.

Authors

Shigeki Shizawa¹, Fujiko Fukuda², Yasuhiro Kikkawa³, Toru Oi⁴, Hitoshi Takemae⁵, Tsuneyuki Masuda⁶, Hiroho Ishida¹, Hironobu Murakami¹, Shoichi Sakaguchi⁷, Tetsuya Mizutani⁵, Makoto Nagai^{1

5}, Mami Oba^{8

9}

Affiliations

¹ School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan.
² Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa, 920-3101, Japan.
³ Global Pig Farm, Inc., Shibukawa, Gunma, 377-0052, Japan.
⁴ Faculty of Bioresources and Environmental Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
⁵ Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
⁶ Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, 794-0085, Japan.
⁷ Department of Microbiology and Infection Control, Faculty of Medicine, Osaka Medical and Pharmaceutical University, Osaka, 569-8686, Japan.
⁸ School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan. [email protected].
⁹ Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan. [email protected].

PMID: 38451342
DOI: 10.1007/s00705-023-05954-9

Abstract

Group A rotavirus (RVA) sequences were detected in 10.8% (23/212) and 20.7% (87/421) of fecal samples collected in 2017-2022 from wild boars and domestic pigs, using next-generation sequencing. Complete genome sequence analysis of one wild boar and 13 domestic pig RVAs revealed that six of them carried the rare H2 NSP5 genotype. Out of the 39 samples for which the NSP5 genotype could be determined, 23 (59.0%) were of genotype H2. H2 porcine RVAs consist exclusively of Japanese porcine RVAs and exhibit sequence diversity in each segment, suggesting that H2 porcine RVAs may have evolved through reassortment within the Japanese pig population.

MeSH terms

Animals
Genomics
Genotype
Japan / epidemiology
Prevalence
Rotavirus* / genetics
Sus scrofa*
Swine

Grants and funding

21K05947/JSPS KAKENHI