Abstract
The transcription factor HAND1 is a critical regulator of cardiac development which is expressed in sub-populations of cardiac progenitors and cardiomyocytes. The transcription factor NKX2-5, in contrast, is expressed more widely in cardiac cells. Here we report the generation of a dual reporter hESC line where the expression of these genes can be simultaneously measured, enabling lineage analysis as well as studies of HAND1 and NKX2-5 gene regulation and protein function. This tool will have wide utility particularly for research on developmental biology and disease modelling.
Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
MeSH terms
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Basic Helix-Loop-Helix Transcription Factors* / genetics
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Basic Helix-Loop-Helix Transcription Factors* / metabolism
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Cell Differentiation
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Cell Line
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Cell Lineage
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Genes, Reporter
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Homeobox Protein Nkx-2.5* / genetics
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Homeobox Protein Nkx-2.5* / metabolism
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Homeodomain Proteins* / genetics
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Homeodomain Proteins* / metabolism
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Human Embryonic Stem Cells* / cytology
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Human Embryonic Stem Cells* / metabolism
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Humans
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Myocytes, Cardiac / cytology
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Myocytes, Cardiac / metabolism
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Transcription Factors* / genetics
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Transcription Factors* / metabolism
Substances
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Homeobox Protein Nkx-2.5
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Transcription Factors
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Homeodomain Proteins
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NKX2-5 protein, human
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helix-loop-helix protein, eHAND
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Basic Helix-Loop-Helix Transcription Factors
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Green Fluorescent Proteins